If the density of a solution does not change no net movement of water then this solution has the same water potential as the tissues that were incubated in it. Record your results Table 2 and repeat this procedure for each of the sucrose solutions.
Density changes can be observed by watching whether a drop of the original solution floats or sinks in the test solution after tissue incubation.
Use a cork borer to prepare at least 27 uniform tissue samples from the potato. Incubate the cores for at least 1. If necessary, add more of the appropriate solution to completely submerge the cores but the final volume in each tube must be the same.
Plant Physiology Biology - Dr. Slowly release a drop of the methylene blue solution from the pipette and note whether the drop of the dye sinks, disperses, or floats to the surface in this solution and subjectively estimate whether it does so rapidly or slowly.
Mix the tubes thoroughly with a vortex mixer. Alternately, for a more accurate measurement of changes in the solution density, a refractometer can be used. Periodically swirl the containers.
The solution gains or looses water depending on the water potential of the tissue. It is assumed that solute movement between tissue and solution is negligible.
Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to dye the sucrose solution, dip a dry probe into methylene blue powder and then mix. Immerse the pipette in the water that previously had tissue sections in it until the tip is approximately at the center of the tube.
This method depends on the change in density in a solution that occurs after a tissue has been immersed in it. Dispense 10 mL of water or a sucrose solution 0. Put two or preferably three potato cores in each solution water or sucrose.
The Chardokov method provides a quick means to determine plant tissue water potentials. Be sure to use a different pipet for each dye stock. Cut them to the same length ca. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel.Chardakov Method for Determining Water Potential.
Background Information: Question: What is the water potential of potato tissue? Hypothesis: Use a cork borer to prepare at least 27 uniform tissue samples from the potato.
Cut them to the same length (ca. cm) with a razor blade and be sure not to include any fragments of the skin. An experiment was conducted so as to investigate and determine the water potential of potato tissue by length method, where by 8 beakers of solutions of different concentration from M M sucrose and distilled water of M were provided, in which one potato strip of cm length immersed in each beaker for about 90 minutes and re-measured, the result showed the change in length.
- Investigation of Water Potential of Potato Tuber Cells Introduction This experiment is to find out the water potential of potato cells. Water potential is the ability of water to move and is represented by the Greek letter Ïˆ (pronounced Â“syÂ”).
LabBench Activity Calculating Water Potential. Water potential is calculated using the following formula: Water potential (Ψ) = pressure potential (Ψ p) + solute potential (Ψ s). Pressure potential (Ψ p).
In a plant cell, pressure exerted by the rigid cell wall that limits further water uptake. Determining the Water Potential of Potato Tuber Cells. Length: words ( double-spaced pages) Rating: - Determining the Concentration of the Cell Sap in Potato Storage Tissue Aim: To determine the concentration of the cell sap in potato storage tissue.
change in weight in my potato tuber cell samples then the water. Lab 1C- Potato/ Water Potential Use a cork borer to cut four cylinders of potato tissue from the potato.
Trim both ends of each cylinder with a scalpel to remove the skin. Cut each cylinder into sections that are Graph: Percent Change in Mass of Potato for each sucrose solution Lab 1 C- Analysis of Results 1.Download